rabbit α cd3  (Novus Biologicals)

Journal: JCI Insight

Article Title: Epstein-Barr virus infection induces tissue-resident memory T cells in mucosal lymphoid tissues

doi: 10.1172/jci.insight.173489

Figure Lengend Snippet: ( A ) CD8 + T cell depletion efficiency in NALT, spleen, and blood of control (blue) and OKT8-treated (orange) animals given as percentage of CD4 – CD3 + T cells ( n = 8–11 animals per group from 2 independent experiments). ( B and C ) EBV viral loads in IU/mg NALT, IU/1 × 10 6 splenocytes, or IU/mL blood and normalized to the mean of the corresponding nondepleted group. ( D ) CD103 depletion efficiency in NALT, spleen, and blood of control (blue) and Ber-OCT3–treated (orange) animals given as percentage of CD8 + T cells ( n = 10–11 animals per group from 2 independent experiments). ( E and F ) EBV viral loads in IU/mg NALT, IU/1x × 10 6 splenocytes, or IU/mL blood and normalized to the mean of the corresponding nondepleted group. ( G ) Representative histogram of Ki-67 expression on CD8 + TEM in spleen and NALT (left) and quantification (right). ( H ) Representative histogram of Ki-67 expression on CD19 + cells in spleen and NALT (left) and quantification (right) ( n = 13 animals per group from 2 independent experiments). * P ≤ 0.05, *** P ≤ 0.001, **** P ≤ 0.0001. ( A – F ) Mann Whitney U test. ( G and H ) Wilcoxon matched-pairs signed rank test; dotted line indicates limit of detection.

Article Snippet: The following were used: rabbit α–human CD20 (SP32, Cell Marque), mouse α–human CD20 (L26, Dako), rabbit α-CD3 (SP7, Diagnostic Biosystem), rabbit α–human CD103 (EPR4166[2], Abcam), rabbit α–mouse Lyve-1 (polyclonal [103-PA50AG], RELIATech GmbH), and rat α–mouse/human PNAd (MECA-79, BioLegend).

Techniques: Control, Expressing, MANN-WHITNEY

Journal: World Journal of Gastrointestinal Oncology

Article Title: Protein tyrosine phosphatase non-receptor II: A possible biomarker of poor prognosis and mediator of immune evasion in hepatocellular carcinoma

doi: 10.4251/wjgo.v16.i9.3913

Figure Lengend Snippet: Immune cell infiltration in high and low protein tyrosine phosphatase non-receptor 2 expression groups. ++: Interleukin (IL)-6 + T-cell, CD3 + T-cell, CD4 + T-cell and CD8 + T-cell infiltration in the high protein tyrosine phosphatase non-receptor 2 (PTPN2) expression group; +: IL-6 + T-cell, CD3 + T-cell, CD4 + T-cell, and CD8 + T-cell infiltration in the low PTPN2 expression group. IL: Interleukin; PTPN2: Protein tyrosine phosphatase non-receptor 2.

Article Snippet: The anti-β-actin primary antibody and rabbit monoclonal anti-CD3 + /CD4 + /CD8 + T-cell α antibody were purchased from Cell Signaling Technology, Inc. Tween 20 and skim milk were purchased from Sangon Biotechnology Ltd. (Shanghai, China).

Techniques: Expressing

Journal: American Journal of Reproductive Immunology

Article Title: Neisseria gonorrhoeae uses cellular proteins CXCL10 and IL8 to enhance HIV‐1 transmission across cervical mucosa

doi: 10.1111/aji.13111

Figure Lengend Snippet: Evaluation of the role of NG and NGIS on the replication and transmission of HIV‐1. A, Dose‐dependent increase in transcription of HIV‐1LTR in the TZM‐bl demonstrated by increased beta‐galactosidase activity by NGIS compared to CM. B, NG per se did not show any stimulation of the HIV‐LTR activity over control media (CM). NGIS induced higher replication and production of virus particles from latently infected cell lines (C) ACH2 cells and (D) U1 cells compared to CM at both 24 h and 7 d. All cell studies were carried out in three independent experiments with three replicates in each and were analyzed using parametric t tests. E, NGIS increased the transmission of HIV‐1 (HIV BAL 300 μL of TCID50 of 10 6 ) across the mucosa as demonstrated by the transmitted virus (HIV p24) and (F) increase in HIV‐Gag mRNA in the tissues. The P ‐value was calculated as significant using unpaired t test of equal variance because of unequal number of biopsy per condition. G, Increased localization of CD3+ cells observed in the subepithelium in NG‐exposed tissues over CM‐exposed tissues. Figure is a representative image at 200× magnification. H, Quantitation of immuno‐stained CD3+ cells showed a statistically significant increase in CD3+ T cells on NG‐exposed tissue compared to CM‐exposed tissues. P ≤ 0.05 was considered as significant in all cases. For the CD3+ tissue stain, experiments were carried out in 2‐3 biopsies from tissues of three donors and non‐parametric paired Wilcoxon signed‐ranked test was used in the analysis of this data

Article Snippet: Images were taken with the Nikon Eclipse E600 microscope using a 20× or 40× oil objective., , Rabbit polyclonal‐α‐CD3 (A0452, Dako, Glostrup, Denmark), mouse polyclonal‐α‐CD68 (M0814, Dako, Glostrup, Denmark), mouse polyclonal‐α‐CD20 (M0755, Dako, Glostrup, Denmark), mouse polyclonal‐α‐DC‐SIGN (551249, BD Technologies, NC, USA), and mouse polyclonal p55 (M3567, Dako, Glostrup, Denmark) were used to stain the tissue slides.

Techniques: Transmission Assay, Activity Assay, Infection, Quantitation Assay, Staining

Journal: American Journal of Reproductive Immunology

Article Title: Neisseria gonorrhoeae uses cellular proteins CXCL10 and IL8 to enhance HIV‐1 transmission across cervical mucosa

doi: 10.1111/aji.13111

Figure Lengend Snippet: Schematic representation of NG‐induced enhanced HIV‐1 transmission across the ecto‐cervical tissues. NG infection of the epithelial surface leads to secretion of IL‐1β, which induces production of epithelial CXCL10 and IL‐8 upon HIV‐1 infection. These cytokines being chemo‐attractants for immune cells cause migration of higher HIV‐1 target CD3+ T cells to the periphery of the epithelium and the intraepithelial region which then fuels the increase in the HIV‐1 replication at the sub‐mucosa and consequently enhances HIV‐1 transmission

Article Snippet: Images were taken with the Nikon Eclipse E600 microscope using a 20× or 40× oil objective., , Rabbit polyclonal‐α‐CD3 (A0452, Dako, Glostrup, Denmark), mouse polyclonal‐α‐CD68 (M0814, Dako, Glostrup, Denmark), mouse polyclonal‐α‐CD20 (M0755, Dako, Glostrup, Denmark), mouse polyclonal‐α‐DC‐SIGN (551249, BD Technologies, NC, USA), and mouse polyclonal p55 (M3567, Dako, Glostrup, Denmark) were used to stain the tissue slides.

Techniques: Transmission Assay, Infection, Migration

Journal: PLoS ONE

Article Title: Fine-Mapping Resolves Eae23 into Two QTLs and Implicates ZEB1 as a Candidate Gene Regulating Experimental Neuroinflammation in Rat

doi: 10.1371/journal.pone.0012716

Figure Lengend Snippet: Spinal cord and brain sections of DA.PVG- Eae23 congenic rats (the two left columns) and DA rats (the two right columns) with the same MAX score. Sections were stained in the following order: spinal cord cross-sections (top row) and brain sections including optic nerve (row 2) were stained with Klüver (KL) and Hämalaun - Eosin (HE), respectively. Congenic rats revealed no signs of CNS inflammation and demyelination while DA rats showed severe loss of myelin and cell infiltration at the sight of the lesion in the spinal cord white matter, as well as at the optic nerve. Staining of the spinal cord sections against CD3 (row 3), CD8 (row 4) and Foxp3 (bottom row) revealed the higher recruitment of CD3 + T cells and CD8 + macrophages respectively to inflammatory lesions in the DA rats and higher recruitment of Foxp3 + T cells in DA.PVG- Eae23 congenic rats. Arrow heads point to cells with positive staining and indicate the relative number of positive cells present.

Article Snippet: Staining was performed with antibodies against the following targets: mouse monoclonal α-CD8 (1∶200, Serotec, UK), rabbit monoclonal α-CD3 (1∶150, Thermo Scientific, UK) and rat monoclonal Foxp3 (1∶1000, eBioscience, UK).

Techniques: Staining